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净信冷冻研磨仪|大鼠皮肤组织研磨

发布时间:2021/02/22 点击量:

  ZAISHENGWUJISHUYIJIYIXUELINGYUZHONG,CHANGCHANGXUYAODUIXIBAOHUOZUZHIJINXINGPOSUICHULICONGERTIQUSUOXUYAODEGEZHONGWUZHI。XIBAOHUOZUZHIPOSUIDEFANGFABAOKUOJIXIEFANGFA、WULIFANGFA、HUAXUEFANGFAHESHENGWUHUAXUEFANGFADENG,QIZHONGJIXIEFAZHUYAOTONGGUOJIXIEQIELIDEZUOYONGSHIZUZHIXIBAOPOSUI,CHANGYONGDEZHUANGZHIYOUZUZHIDAOSUIJI、YUNJIANGQI、CHAOSHENGPOSUIYIDENG。  

  ZUZHIDAOSUIJIYIBANYONGYUDONGWUZUZHI、ZHIWUROUZHIZHONGZI、ROUNENDEYEYADENG,QIZHUANSUKEGAODA 10000rpm/MYISHANG,YOUYUXUANZHUANDAOPIANDEJIXIEQIELIHENDA,ZHIBEIYIXIEJIAODAFENZIRUHESUANZEHENSHAOSHIYONG。 GAOYAYUNJIANGQISHIYOUKECHANSHENGGAOYADEZHENGXIANGPAIDAIBENGHEPAICHUFAZUCHENG,PAICHUFAJUYOUXIAZHAIDEXIAOKONG,XIBAOJIANGYETONGGUOZHINIFAJINRUBENGTINEI,ZAIGAOYAXIAPOSHIQIZAIPAICHUFADEXIAOKONGZHONGGAOSUCHONGCHU,BINGSHEXIANGZHUANGJIHUANSHANG,YOUYUTURANJIANYAHEGAOSUCHONGJI,SHIXIBAOSHOUDAOGAODEYEXIANGJIANQIELIERPOSUI,CIFASHIYONGYUXUDUOWEISHENGWUXIBAODEPOSUI。CHAOSHENGPOSUIYIJIANGDIANNENGTONGGUOHUANNENGQIZHUANHUANWEISHENGNENG,ZHEIZHONGNENGLIANGTONGGUOYETIJIEZHIERBIANCHENGYIGEGEMIJIDEXIAOQIPAO,XIAOQIPAOXUNSUZHALIECHANSHENGDENENGLIANGQIDAOPOSUIXIBAODENGWUZHIDEZUOYONG。  

  PIFUZUZHISHIYOUBIAOPI、ZHENPIHEPIXIAZUZHISANBUFENZUCHENG。QIZHONG,BIAOPIDEJIAOZHICENGCHUZAIZUIWAIMIAN,JUYOURENXING,NENGGOUNAISHOUYIDINGDEMOCA;ERZHENPINEIBUDEXIBAOHENSHAO,ZHUYAOYOUZHIWUXIANWEIJIEDIZUZHIGOUCHENG,QIZHONGYOUJIAOYUANXIANWEI、DANXINGXIANWEIHEWANGZHUANGXIANWEIDENG,FUYUPIFUYIDINGDEDANXING HEZHANGLI。PIFUZUZHIDEZHEIXIETEXINGJUEDINGLESHANGSHUCHANGYONGDEYANMOFANGSHIJUNBUSHIYONGYUPIFUZUZHIDEPOSUI。YANBOSHISHIYANSHIZHONGCHANGYONGDEYANMOGONGJU,QIPEIYOUBOCHU,TONGGUOBOCHUYUYANBODEJIYAYUMOCALAIDADAODAOMO、YANMO、POSUIDENGMUDE,QISUINENGGOUJIAOWEIYOUXIAODIPOSUIPIFUZUZHI,RANERXUYAOJINXINGSHOUGONGCAOZUO,FEISHIFEILI。

 样品研磨前

  大鼠的皮肤比较厚,因此用上面方法完全研磨这些组织是十分具有挑战性的,使用上海净信冷冻研磨仪,成功实现了对大鼠后爪蛋白质和纸质的研磨提取。  

实验方法:  

  1、QUDASHUPIFUZUZHIRUOGAN。  

  2、JIAHUANCHONGYEHEJINGXINZHUANYONGPIFUYANMOZHU。  

  3、FANGRUJINGXINLENGDONGYANMOYIZHONG。  

  4、DIAOHAOSHIJIANPINLV。  

  5、DEDAOWANMEIPOSUIRONGYE。 

  6、TIXINTIQUSHANGQINGRONGYE。

样品研磨前

  从本实验后续实验人员反馈情况来看,用上海净信冷冻研磨仪和研钵对比来看,优势有这样几个。1、研磨后样品无交叉污染。2、样品后续RNA得率很高。3、研磨样品节省了液氮,降低实验室成本。4、研磨时间得到大大提高,以前几个人工作量减少到一个人几分钟完成批量样品,大大提高了效率。

冷冻研磨仪

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